Polyaniline-dacron composite as solid phase in enzyme linked immunosorbent assay forYersinia pestis antibody detection

Abstract In this enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against extractable nuclear antigens (ENA) in sera of patients with systemic lupus erythematosus (SLE), nylon is used as solid phase for antigen binding instead of the commonly used polystyrene surface. Optimal conditions for activation of the nylon beads, antigen coating, and other relevant factors have been investigated. We compared the incidence of anti-ENA antibodies in SLE, using chromogenic and fluorogenic enzyme substrates. Of SLE patients, 54% were positive for anti-ENA antibodies when chromogenic substrate was used as compared with 68% for fluorogenic substrate. Antibody activity against Sm and RNP antigens was distinguished on the basis of ribonuclease sensitivity of the RNP antigen. The method described offers advantages such as decreased background activity, increased surface area, facility for prolonged storage of antigen-coated solid phase, and miniaturization of the assay.

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Polyvinyl alcohol-glutaraldehyde as solid-phase in Elisa for Schistosomiasis

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46651997000300006 ◽

1997 ◽

Vol 39 (3) ◽

pp. 155-158 ◽

Cited By ~ 6

Author(s):

Aureci M. ARAÚjO ◽

Gustavo H.T.S. BARBOSA ◽

José Ricardo P. DINIZ ◽

Elizabeth MALAGUEÑO ◽

Walter M. AZEVEDO ◽

...

Keyword(s):

Polyvinyl Alcohol ◽

Schistosoma Mansoni ◽

Immunosorbent Assay ◽

Reliable Method ◽

Solid Phase ◽

Enzyme Linked Immunosorbent Assay ◽

Antigen Preparation ◽

Conventional Elisa ◽

Set Up ◽

As Solid Phase

Soluble adult Schistosoma mansoni antigen preparation (SWAP) was covalently fixed onto polyvinyl alcohol-glutaraldehyde discs and an enzyme linked-immunosorbent assay (ELISA) was set up. The best conditions for the assay were established and it was found that small amount of antigen such as 1.5 µg was required. A comparison between this procedure and the conventional ELISA was proceeded. A reliable method of antigen immobilization was achieved and the low prices of the employed reagents are economically attractive

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Enzyme-linked immunosorbent assay for serum amyloid A apolipoprotein with use of specific antibodies against synthetic peptides.

Clinical Chemistry ◽

10.1093/clinchem/34.9.1763 ◽

1988 ◽

Vol 34 (9) ◽

pp. 1767-1771 ◽

Cited By ~ 9

Author(s):

R Saïle ◽

C Delpierre ◽

P Puchois ◽

G Hocke ◽

C Cachera ◽

...

Keyword(s):

Immunosorbent Assay ◽

Synthetic Peptides ◽

Solid Phase ◽

Polyclonal Antibodies ◽

Enzyme Linked Immunosorbent Assay ◽

Specific Antibodies ◽

Amyloid A ◽

Microtiter Plates ◽

Sensitivity Specificity ◽

As Solid Phase

Abstract We describe a noncompetitive enzyme-linked immunosorbent assay for amyloid A apolipoprotein in human serum (apo SAA) in which specific antibodies against synthetic peptides are used. Microtiter plates were used as solid phase and coated with affinity-purified antibodies raised against SAA1-(95-104) peptide. After incubation with delipidated plasmas, the bound apo SAA was revealed by labeled antibodies raised against SAA1-(58-69) peptide. The assay offers several advantages over existing techniques: sensitivity, specificity, simplicity, and non-use of radioisotopes. Results correlate well with those by a nephelometric method in which polyclonal antibodies are used.

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Dot enzyme-linked immunosorbent assay (dot-ELISA) for schistosomiasis diagnosis using dacron as solid-phase

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86821999000200004 ◽

1999 ◽

Vol 32 (2) ◽

pp. 139-143 ◽

Cited By ~ 6

Author(s):

Silvia Maria Lucena Montenegro ◽

Joanne D'arc Bezerra da Silva ◽

Maria Edileuza Felinto de Brito ◽

Luiz Bezerra de Carvalho Junior

Keyword(s):

Immunosorbent Assay ◽

Solid Phase ◽

Population Surveillance ◽

Enzyme Linked Immunosorbent Assay ◽

Healthy Individuals ◽

Antigen Preparation ◽

Endemic Areas ◽

The Difference ◽

Dot Elisa ◽

As Solid Phase

Dacron and nitrocellulose were evaluated as matrices for the dot enzyme linked immunosorbent assay (dot-ELISA) for schistosomiasis and compared to indirect immunofluorescence (IMF). Titration of sera from 18 schistosomiasis patients against soluble worm antigen preparation (SWAP) was carried out and sera from healthy individuals from non-endemic areas were used as controls. The IMF was less sensitive than the dot-ELISAs, although the difference was not statistically significant (p > 0.05). The dot-ELISA based on nitrocellulose was as sensitive as that using dacron. Stability did not differ between nitrocellulose and dacron. Specificity was lower when dacron was used than when nitrocellulose was used, although the difference was not statistically significant (p > 0.05). In conclusion, this work showed that nitrocellulose and dacron performed similarly in dot-ELISA, suggesting that they may be used alternatively in population surveillance in endemic areas.

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